Studies of Paroxysmal Nocturnal Hemoglobinuria Erythrocytes: Increased Lysis and Lipid Peroxide Formation by Hydrogen Peroxide*
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چکیده
منابع مشابه
Studies of paroxysmal nocturnal hemoglobinuria erythrocytes: increased lysis and lipid peroxide formation by hydrogen peroxide.
When paroxysmal nocturnal hemoglobinuria (PNH) erythrocytes were exposed to H(2)O(2) they lysed excessively and formed greater than normal quantities of lipid peroxides when compared to red cells of normal subjects and patients with most types of hematologic disease. It was also shown that lytic sensitivity to acidified serum was related to the enhanced lytic sensitivity to H(2)O(2). If the lip...
متن کاملLeukocyte antigen-antibody reaction and lysis of paroxysmal nocturnal hemoglobinuria erythrocytes.
T HE TRANSFUSION OF COMPATIBLE WHOLE BLOOD to patients suffering from paroxysmal nocturnal hemoglobinuria ( PNH ) is known to sometimes cause a hemolytic reaction. It has been suggested that leukocyte isoantibodies present in the patient’s serum through interaction with the donor’s leukocyte antigens are responsible for lysis of PNH red cells.’ As yet, it has not been clarified why this interac...
متن کاملEnhanced reactive lysis of paroxysmal nocturnal hemoglobinuria erythrocytes. Studies on C9 binding and incorporation into high molecular weight complexes
As part of a broader analysis of the mechanism(s) by which the most sensitive (type III) paroxysmal nocturnal hemoglobinuria (PNH) erythrocytes are excessively sensitive to reactive lysis by isolated C5b6, C7, C8, and C9, we have compared type III PNH (PNH-III) and normal human E in respect to both total specific binding of 125I-C9 and the proportion of cell-bound C9 appearing in high molecular...
متن کاملCharacterization of the complement sensitivity of paroxysmal nocturnal hemoglobinuria erythrocytes.
The affected erythrocytes of paroxysmal nocturnal hemoglobinuria (PNH II and PNH III cells) are abnormally sensitive to complement-mediated lysis. Normal human erythrocytes chemically modified by treatment with 2-amino-ethylisothiouronium bromide (AET) have been used as models for PNH cells inasmuch as they also exhibit an enhanced susceptibility to complement. To investigate the bases for the ...
متن کاملAbnormality of glycophorin-alpha on paroxysmal nocturnal hemoglobinuria erythrocytes.
To investigate the greater enzymatic activity of the alternative pathway convertase (and the subsequent greater fixation of C3b) on paroxysmal nocturnal hemoglobinuria (PNH) erythrocytes, we have examined the topography of binding of C3b to PNH and normal erythrocytes. Using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography, the alpha-chain of C3b was found to bind v...
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ژورنال
عنوان ژورنال: Journal of Clinical Investigation
سال: 1967
ISSN: 0021-9738
DOI: 10.1172/jci105662